Raft Protein Clustering Alters N-Ras Membrane Interactions and Activation Pattern
نویسندگان
چکیده
منابع مشابه
TCR-induced activation of Ras proceeds at the plasma membrane and requires palmitoylation of N-Ras.
Ras transmits manifold signals from the TCR at various crossroads in the life of a T cell. For example, selection programs in the thymus or the acquisition of a state of hypo-responsiveness known as anergy are just some of the T cell features known to be controlled by TCR-sparked signals that are intracellularly propagated by Ras. These findings raise the question of how Ras can transmit such a...
متن کاملThe deubiquitinating enzyme USP17 blocks N-Ras membrane trafficking and activation but leaves K-Ras unaffected.
The proto-oncogenic Ras isoforms (H, N, and K) have a C-terminal CAAX motif and undergo the same post-translational processing steps, although they traffic to the plasma membrane through different routes. Previously, we have shown that overexpression of the deubiquitinating enzyme USP17 inhibits H-Ras localization to the plasma membrane. Now we report that whereas H-Ras and N-Ras were unable to...
متن کاملIdentifying optimal lipid raft characteristics required to promote nanoscale protein-protein interactions on the plasma membrane.
The dynamic lateral segregation of signaling proteins into microdomains is proposed to facilitate signal transduction, but the constraints on microdomain size, mobility, and diffusion that might realize this function are undefined. Here we interrogate a stochastic spatial model of the plasma membrane to determine how microdomains affect protein dynamics. Taking lipid rafts as representative mic...
متن کاملClustering induces redistribution of syndecan-4 core protein into raft membrane domains.
Syndecan-4 is a heparan sulfate-carrying core protein that has been directly implicated in fibroblast growth factor 2 (FGF2) signaling. Recent studies have suggested that many signaling proteins localize to the raft compartment of the plasma cell membrane. To establish whether syndecan-4 is present in the raft compartment, we have studied the distribution of the core protein and an Fc receptor ...
متن کاملToward understanding the dynamics of membrane-raft-based molecular interactions.
The cell membrane is a 2-dimensional non-ideal liquid containing dynamic structures on various time-space scales, and the raft domain is one of them. Existing literature supports the concept that raft dynamics may be important for its formation and function: the raft function may be supported by stimulation-induced raft association/coalescence and recruitment of various raftophilic molecules to...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 2011
ISSN: 0270-7306
DOI: 10.1128/mcb.05570-11